Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the expression of metastasis-related factors via the NFκB pathway. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. a Protein levels of MTA1; cells were collected for WB with MTA1 and GAPDH antibodies. The bands of over-expressed MTA1 were from Additional file : Fig. 2A and down-expressed MTA1 were from Additional file : Fig. 2B with arrow. b Cells were lysed and used in qRT-PCR assays to measure the E-cadherin mRNA expression. Statistical analysis was performed using Student’s t test. b , c A498 cells were transfected with pcDNA3.1-Flag and Flag-MTA1. After 36 h, the Flag-MTA1 group was treated with 10 nM PDTC for 12 h. Then cells were harvested for qRT-PCR evaluation to measure the mRNA expression of MMP2 ( b ) and MMP9 ( c ). Statistical analysis was performed using ANOVA. d A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 48 h, cells were subjected to western blotting analysis using antibodies targeting p-p65, p65 and ACTB. The bands of p-p65, p65 and ACTB were from Additional file : Fig. 3. e, f Image J was used to calculate the gray scanned bands of p-p65 ( b ) and p65 (C). * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; NS not significant

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 promotes A498 cell migration. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, cells in the four conditions were subjected to the wound healing assay. Macrographs were taken under ×100 magnification

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 enhances the invasion of A498 cells. a A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, the transwell cell invasion assay using A498 cells was performed, and macrographs were taken under ×40 magnification. Scale bar: 50 µm. b Image J was used to count the transmigrated cells and Student’s t test to analyze differences. ** p < 0.01; *** p < 0.001

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Transfection, Invasion Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the migration and invasion of RCC cells via NF-κB. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, and Flag-MTA1 + PDTC (10 nM). a In the wound healing assay, MTA1 improved the migration of RCC cells, while the addition of the inhibitor PDTC blocked the effect of MTA1 on migration. Scale bar: 100×. b In the transwell assay, MTA1 markedly induced invasion of A498 cells, but compared to the MTA1 group, the invasion of MTA1 + PDTC A498 cells was lower. ** p < 0.01; *** p < 0.001; Scale bar: ×40

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay, Transwell Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: The expression of MTA1 is up-regulated in RCCs. a Immunohistochemistry was used to characterize the expression of MTA1 in RCC and adjacent tissues. MTA1 was highly expressed in ccRCCs, compared to very weak staining in adjacent tissue. b Image Pro Plus was used for the statistical analysis of the positive signal (IOD) of MTA1 expression in ccRCCs and the adjacent tissue. The expression of MTA1 was significantly up-regulated in ccRCCs. ** p < 0.01; scale bar: 100 µm and 50 µm. c The expression of MTA1 in RCC cell lines. Normal renal cell line HEK293T and RCC cell lines A498 and 768-O cell lysates were used in western blotting analysis with MTA1 and GAPDH antibodies. The bands of MTA1 were from Additional file : Fig. 1 and gapdh were from Additional file : Fig. 1

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: Molecular Oncology

Article Title: Obesity alters the fitness of peritumoral adipose tissue, exacerbating tumor invasiveness in renal cancer through the induction of ADAM12 and CYP1B1

doi: 10.1002/1878-0261.13782

Figure Lengend Snippet: Adipose peritumoral tissue affects the expression of inflammatory genes by renal cell carcinoma (RCC) cells. (A) Relative mRNA expression of the different tumor suppressor genes in the healthy and tumor kidney tissues of RCC patients ( n = 10), including VHL , PBRM1 , SETD2 , and BAP1 , was measured by real‐time PCR. Cyclophilin‐A was used as an internal control. Data are shown as mean ± SD from at least 3 separate experiments. P ‐values were determined by Student's t ‐test. (B) Schematic figure illustrating the protocol for differentiation of adipocytes from peritumoral adipose tissue (AT) of lean, overweight, and obese RCC patients. (C) Relative mRNA expression of the leptin and adiponectin of the differentiated adipocytes among the lean, overweight, and obese groups ( n = 6). Data are shown as mean ± SD from at least 3 separate experiments. P ‐values were determined by one‐way ANOVA. (D) Schematic figure of the conditioned medium (CM) experiment, showing RCC cells treated with conditioned media (CMs) of lean, overweight, and obese differentiated adipocytes. (E) Relative mRNA expression of the IL6 , CXCR4 , SDF1 , and BAFFR of the A498 cells treated with CMs collected from differentiated adipocytes derived from the lean, overweight, and obese groups ( n = 6). Data are shown as mean ± SD from at least 3 separate experiments. P ‐values were determined by one‐way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The human renal cancer cell line (A498) was grown in DMEM (Microgem); supplemented with 10% FBS, 1% Pen/Strep, and 1% L‐Glutamine, at 37 °C in 5% CO 2 .

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Derivative Assay

Journal: Oncology Reports

Article Title: Downregulation of microRNA-15a suppresses the proliferation and invasion of renal cell carcinoma via direct targeting of eIF4E

doi: 10.3892/or.2017.5901

Figure Lengend Snippet: Expression of miR-15a is downregulated in RCC tissues and cell lines. (A) qRT-PCR detection of miR-15a expression in 40 RCC specimens and adjacent normal tissues. (B) qRT-PCR detection of miR-15a expression in 4 RCC cell lines and normal renal cell line HK-2. (C) Correlation between miR-15a and eIF4E mRNA in RCC tissues using linear correlation. Each experiment was repeated at least 3 times; *P<0.05, **P<0.01 vs. the control group.

Article Snippet: The human renal carcinoma cell lines (ACHN, 786-O, 769-P and OS-RC-2) and normal renal cell line HK-2 were obtained from the China Center for Type Culture Collection (CCTCC; Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Control